CRY1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-69080

Novus Biologicals, part of Bio-Techne
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NBP1-69080
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Key Product Details

Species Reactivity

Validated:

Human, Amphibian

Cited:

Syrian Hamster

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all CRY1 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to CRY1 (cryptochrome 1, photolyase-like). The peptide sequence was selected from the N terminal of CRY1 (NP_004066). Peptide sequence KRFQTLISKMEPLEIPVETITSEVIEKCTTPLSDDHDEKYGVPSLEELGF. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Amphibian reactivity reported in a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for CRY1 Antibody

Western Blot: CRY1 Antibody [NBP1-69080]

Western Blot: CRY1 Antibody [NBP1-69080]

Western Blot: CRY1 Antibody [NBP1-69080] - Sample Tissue: Hela Whole cell, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane, Gel Concentration: 0.12%
Immunohistochemistry-Paraffin: CRY1 Antibody [NBP1-69080]

Immunohistochemistry-Paraffin: CRY1 Antibody [NBP1-69080]

Immunohistochemistry-Paraffin: CRY1 Antibody [NBP1-69080] - Human testis tissue at an antibody concentration of 5ug/ml.
Western Blot: CRY1 Antibody [NBP1-69080]

Western Blot: CRY1 Antibody [NBP1-69080]

Western Blot: CRY1 Antibody [NBP1-69080] - Sample Tissue:Hela Whole cell. Lane A: Primary Antibody Lane B: Primary Antibody + Blocking.
View 1 more images

Applications for CRY1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

Validated for Immunohistochemistry-Frozen from a verified customer review.

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

reported by internal validation

Western Blot

1.0 ug/ml
Application Notes
In Simple Western internal validation: HeLa lysate at 0.5 mg/ml as sample; separated by size; antibody dilution of 1:50; observed molecular weight was 61 kDa;matrix was 12-230 kDa; detected by Chemiluminescence.

Reviewed Applications

Read 1 review rated 4 using NBP1-69080 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRY1

CRY1 is the blue light-dependent regulator of the circadian feedback loop. 'CRY1 inhibits CLOCK|NPAS2-ARNTL E box-mediated transcription.'CRY1 acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. 'CRY1 has no photolyase activity. 'CRY1 is capable of translocating circadian clock core proteins such as PER proteins to the nucleus. 'CRY1 may inhibit CLOCK|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.

Long Name

Cryptochrome 1

Alternate Names

Cryptochrome I, PHLL1

Entrez Gene IDs

1407 (Human)

Gene Symbol

CRY1

UniProt

Q16526

Additional CRY1 Products

Product Documents for CRY1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CRY1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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