CRY2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86273

Novus Biologicals, part of Bio-Techne
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NBP1-86273
NBP1-86273-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CRY2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLASVPSCVEDLSHPVAEPSSSQAGSMSSAGPRPLPSGPASPKRKLEAAEEPPGEELSKRARVAELPTPELPSKDA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRY2 Antibody

Western Blot: CRY2 Antibody [NBP1-86273]

Western Blot: CRY2 Antibody [NBP1-86273]

Western Blot: CRY2 Antibody [NBP1-86273] - Analysis in control (vector only transfected HEK293T lysate) and CRY2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: CRY2 Antibody [NBP1-86273]

Immunocytochemistry/ Immunofluorescence: CRY2 Antibody [NBP1-86273]

Immunocytochemistry/Immunofluorescence: CRY2 Antibody [NBP1-86273] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.
Immunohistochemistry-Paraffin: CRY2 Antibody [NBP1-86273]

Immunohistochemistry-Paraffin: CRY2 Antibody [NBP1-86273]

Immunohistochemistry-Paraffin: CRY2 Antibody [NBP1-86273] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Applications for CRY2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRY2

Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK NPAS2-ARNTL E box-mediatedtranscription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has nophotolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. Mayinhibit CLOCK NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL

Long Name

Cryptochrome-2

Alternate Names

KIAA0658

Gene Symbol

CRY2

Additional CRY2 Products

Product Documents for CRY2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CRY2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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