CRYBA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33010

Novus Biologicals, part of Bio-Techne
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NBP1-33010
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Predicted:

Bovine (93%), Canine (96%), Guinea Pig (95%), Rabbit (96%), Rat (95%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CRYBA1 Antibodies »

Product Specifications

Immunogen

Full length human beta A3 Crystallin Recombinant protein.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 31488069).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CRYBA1 Antibody

Western Blot: CRYBA1 Antibody [NBP1-33010]

Western Blot: CRYBA1 Antibody [NBP1-33010]

Western Blot: CRYBA1 Antibody [NBP1-33010] - The CRYBA1 p.G91del mutation reduced its expression in two cell lines. The protein levels of CRYBA1 were measured by Western blot. In 293T and SRA cells, the exogenous CRYBA1 was detected using anti-FLAG antibody; In SRA cells, the general CRYBA1 protein level was also measured using anti-CRYBA1 antibody. beta-Actin or GAPDH were used as internal control. NC, negative control: transfection reagent only. Image collected and cropped by CiteAb from the following publication (bmcmedgenet.biomedcentral.com/articles/10.1186/s12881-019-0882-z), licensed under a CC-BY license.
Western Blot: CRYBA1 Antibody [NBP1-33010]

Western Blot: CRYBA1 Antibody [NBP1-33010]

Western Blot: CRYBA1 Antibody [NBP1-33010] - A. 50 ug mouse fetal eye lysate/extract 12 % SDS-PAGE beta A3 Crystallin antibody dilution: 1:10000
CRYBA1 Antibody

Immunocytochemistry/ Immunofluorescence: CRYBA1 Antibody [NBP1-33010] -

Immunocytochemistry/ Immunofluorescence: CRYBA1 Antibody [NBP1-33010] - Immunofluorescence staining of exogenous WT & mutated CRYBA1 in 2 cell lines. After the WT & deleted forms of CRYBA1 cDNA constructs were transfected into 293 T & SRA lines, cells were stained with FLAG primary antibody. p.G91del mutation led to greater aggregation of the protein at the cell membrane. Blue: nuclear; green: exogenous CRYBA1, scale bar: 20 μM Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31488069), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
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Applications for CRYBA1 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:31488069).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CRYBA1

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'.

Alternate Names

CRYB1beta-crystallin A3, crystallin, beta A1, crystallin, beta A3, eye lens structural protein

Gene Symbol

CRYBA1

Additional CRYBA1 Products

Product Documents for CRYBA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CRYBA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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