CSK Antibody (1A2F5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15297

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15297-100ul
NBP3-15297-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1A2F5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CSK Antibodies »

Product Summary for CSK Antibody (1A2F5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CSK (P41240). MSAIQAAWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CSK Antibody (1A2F5)

Western Blot: CSK Antibody (1A2F5) [NBP3-15297]

Western Blot: CSK Antibody (1A2F5) [NBP3-15297]

Western Blot: CSK Antibody (1A2F5) [NBP3-15297] - Western blot analysis of extracts of various cell lines, using CSK Rabbit mAb (NBP3-15297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: CSK Antibody (1A2F5) [NBP3-15297]

Immunohistochemistry-Paraffin: CSK Antibody (1A2F5) [NBP3-15297]

Immunohistochemistry-Paraffin: CSK Antibody (1A2F5) [NBP3-15297] - Immunohistochemistry of paraffin-embedded mouse spleen using CSK Rabbit mAb (NBP3-15297) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: CSK Antibody (1A2F5) [NBP3-15297]

Immunohistochemistry-Paraffin: CSK Antibody (1A2F5) [NBP3-15297]

Immunohistochemistry-Paraffin: CSK Antibody (1A2F5) [NBP3-15297] - Immunohistochemistry of paraffin-embedded rat liver using CSK Rabbit mAb (NBP3-15297) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for CSK Antibody (1A2F5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CSK

Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on theLYN and FYN kinases

Long Name

Tyrosine-protein kinase CSK

Alternate Names

C-Src kinase

Gene Symbol

CSK

Additional CSK Products

Product Documents for CSK Antibody (1A2F5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CSK Antibody (1A2F5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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