CSK Antibody (3A3)

Novus Biologicals, part of Bio-Techne | Catalog # H00001445-M01

Novus Biologicals, part of Bio-Techne
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H00001445-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3A3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CSK Antibodies »

Product Specifications

Immunogen

CSK (NP_004374, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSAIQAAWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPE

Specificity

CSK - c-src tyrosine kinase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CSK Antibody (3A3)

Western Blot: CSK Antibody (3A3) [H00001445-M01]

Western Blot: CSK Antibody (3A3) [H00001445-M01]

Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody (M01), clone 3A3.Lane 1: CSK transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: CSK Antibody (3A3) [H00001445-M01]

Immunohistochemistry-Paraffin: CSK Antibody (3A3) [H00001445-M01]

Immunohistochemistry-Paraffin: CSK Antibody (3A3) [H00001445-M01] - Analysis of monoclonal antibody to CSK on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.
Western Blot: CSK Antibody (3A3) [H00001445-M01]

Western Blot: CSK Antibody (3A3) [H00001445-M01]

Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK over-expressed 293 cell line, cotransfected with CSK Validated Chimera RNAi ( Cat # H00001445-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSK monoclonal antibody (M01) clone 3A3 (Cat # H00001445-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for CSK Antibody (3A3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CSK

Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on theLYN and FYN kinases

Long Name

Tyrosine-protein kinase CSK

Alternate Names

C-Src kinase

Entrez Gene IDs

1445 (Human)

Gene Symbol

CSK

OMIM

124095 (Human)

UniProt

NP_004374 (Human)

Additional CSK Products

Product Documents for CSK Antibody (3A3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CSK Antibody (3A3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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