Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CSK-04

Format

BSA Free

Concentration

1.0 mg/ml

View all CSK Antibodies »

Product Specifications

Immunogen

Bacterially expressed recombinant fragment of human Csk corresponding to amino acids 330-450.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Nuclear

Specificity

The antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa non-receptor protein tyrosine kinase expressed in cytosol.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CSK Antibody (CSK-04) - BSA Free

Immunocytochemistry/ Immunofluorescence: CSK Antibody (CSK-04) - BSA Free [NB500-344]

Immunocytochemistry/ Immunofluorescence: CSK Antibody (CSK-04) - BSA Free [NB500-344]

Immunocytochemistry/Immunofluorescence: CSK Antibody (CSK-04) [NB500-344] - Staining of Csk in human HeLa cell line using anti-Csk (CSK-04; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Applications for CSK Antibody (CSK-04) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:50

Western Blot

1:200-1:300
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CSK

C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases that are localized in lipid rafts is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.

Long Name

Tyrosine-protein kinase CSK

Alternate Names

C-Src kinase

Entrez Gene IDs

1445 (Human); 12988 (Mouse)

Gene Symbol

CSK

Additional CSK Products

Product Documents for CSK Antibody (CSK-04) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CSK Antibody (CSK-04) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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