CSK Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92944

Novus Biologicals, part of Bio-Techne
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NBP2-92944-0.02ml
NBP2-92944-0.1ml
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CSK Antibodies »

Product Summary for CSK Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CSK (NP_001120662.1). MSAIQAAWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCDGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTSDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CSK Antibody - BSA Free

Western Blot: CSK AntibodyBSA Free [NBP2-92944]

Western Blot: CSK AntibodyBSA Free [NBP2-92944]

Western Blot: CSK Antibody [NBP2-92944] - Western blot analysis of extracts of various cell lines, using CSK antibody (NBP2-92944) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: CSK Antibody - BSA Free [NBP2-92944]

Immunocytochemistry/ Immunofluorescence: CSK Antibody - BSA Free [NBP2-92944]

Immunocytochemistry/Immunofluorescence: CSK Antibody [NBP2-92944] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] CSK Rabbit pAb (NBP2-92944) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: CSK Antibody - BSA Free [NBP2-92944]

Immunohistochemistry-Paraffin: CSK Antibody - BSA Free [NBP2-92944]

Immunohistochemistry-Paraffin: CSK Antibody [NBP2-92944] - Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] CSK Rabbit pAb (NBP2-92944) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for CSK Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CSK

Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on theLYN and FYN kinases

Long Name

Tyrosine-protein kinase CSK

Alternate Names

C-Src kinase

Gene Symbol

CSK

Additional CSK Products

Product Documents for CSK Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CSK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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