CSRP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06983

Novus Biologicals, part of Bio-Techne
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NBP1-06983
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CSRP3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CGKSLESTNVTDKD corresponding to internal region according to NP_003467.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CSRP3 Antibody

Western Blot: CSRP3 Antibody [NBP1-06983]

Western Blot: CSRP3 Antibody [NBP1-06983]

Western Blot: CSRP3 Antibody [NBP1-06983] - (0.01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983]

Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983]

Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983]

Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983]

Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for CSRP3 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01-0.03 ug/ml
Application Notes
Western blot: Approx. 20kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 21.0kDa according to Human NP_003467.1 and 20.8kDa according to Rat NP_476485.1). Primary incubation was 1 hour.
IHC: Paraffin embedded Human Skeletal Muscle and Heart.
Use in Immunocytochemistry/immunofluorescence and Immunoprecipitation reported in scientific literature PMID: 27551448.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CSRP3

This gene encodes a member of the CSRP family of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this protein is found in a group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Mutations in this gene are thought to cause heritable forms of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) in humans. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq]

Alternate Names

Cardiac LIM protein, CLPMGC14488, CMD1Mcardiac LIM domain protein, CMH12, CRP3cysteine and glycine-rich protein 3, cysteine and glycine-rich protein 3 (cardiac LIM protein), Cysteine-rich protein 3, LIM domain only 4, LIM domain protein, cardiac, LMO4, MLPMGC61993, Muscle LIM protein

Entrez Gene IDs

8048 (Human); 13009 (Mouse); 117505 (Rat)

Gene Symbol

CSRP3

UniProt

NP_003467.1 (Human)

Additional CSRP3 Products

Product Documents for CSRP3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CSRP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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