CSTF3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57534

Novus Biologicals, part of Bio-Techne
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NB100-57534
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all CSTF3 Antibodies »

Product Summary for CSTF3 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 667 and 717 of human cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa using the numbering given in entry NP_001317.1 (GeneID 1479).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CSTF3 Antibody

Western Blot: CSTF3 Antibody [NB100-57534]

Western Blot: CSTF3 Antibody [NB100-57534]

Western Blot: CSTF3 Antibody [NB100-57534] - Detection of Human and Mouse CSTF77 on HeLa whole cell lysate using NB100-57534. CSTF77 was also immunoprecipitated by rabbit anti-CSTF77 antibodies NB100-57532 and NB100-57533.
Immunohistochemistry: CSTF3 Antibody [NB100-57534]

Immunohistochemistry: CSTF3 Antibody [NB100-57534]

Immunohistochemistry: CSTF3 Antibody [NB100-57534] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CSTF77 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for CSTF3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CSTF3

The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

CF-1 77 kDa subunit, Cleavage stimulation factor 77 kDa subunit, cleavage stimulation factor subunit 3, cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kD, cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa, CSTF 77 kDa subunit, CSTF-77, MGC117398, MGC43001, MGC75122

Entrez Gene IDs

1479 (Human)

Gene Symbol

CSTF3

UniProt

NP_001317.1 (Human)

Additional CSTF3 Products

Product Documents for CSTF3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CSTF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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