CTBP2 Antibody (1R1U6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15268
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1R1U6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CTBP2 Antibody (1R1U6)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 346-445 of human CTBP2 (P56545). TGRIPESLRNCVNKEFFVTSAPWSVIDQQAIHPELNGATYRYPPGIVGVAPGGLPAAMEGIIPGGIPVTHNLPTVAHPSQAPSPNQPTKHGDNREHPNEQ
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CTBP2 Antibody (1R1U6)
Western Blot: CTBP2 Antibody (1R1U6) [NBP3-15268]
Western Blot: CTBP2 Antibody (1R1U6) [NBP3-15268] - Analysis of extracts of various cell lines, using CTBP2 antibody (NBP3-15268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunohistochemistry-Paraffin: CTBP2 Antibody (1R1U6) [NBP3-15268]
Immunohistochemistry-Paraffin: CTBP2 Antibody (1R1U6) [NBP3-15268] - Rat ovary using CTBP2 Rabbit mAb (NBP3-15268) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Immunocytochemistry/ Immunofluorescence: CTBP2 Antibody (1R1U6) [NBP3-15268]
Immunocytochemistry/Immunofluorescence: CTBP2 Antibody (1R1U6) [NBP3-15268] - Immunofluorescence analysis of NIH/3T3 cells using CTBP2 Rabbit mAb (NBP3-15268) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for CTBP2 Antibody (1R1U6)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 -1:200
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CTBP2
Alternate Names
CtBP2, C-terminal binding protein 2, 10ribeye, C-terminal-binding protein 2
Gene Symbol
CTBP2
Additional CTBP2 Products
Product Documents for CTBP2 Antibody (1R1U6)
Product Specific Notices for CTBP2 Antibody (1R1U6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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