CTCF Antibody (CL0307) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44027
![CTCF Antibody (CL0307) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: CTCF Antibody [NBP3-44027]](https://resources.bio-techne.com/images/products/nbp3-44027_mouse-ctcf-mab-cl0307-azide-and-bsa-free-28220251339125.jpg "Immunocytochemistry/ Immunofluorescence: CTCF Antibody [NBP3-44027]")
![CTCF Antibody (CL0307) - Azide and BSA Free Western Blot: CTCF Antibody [NBP3-44027]](https://resources.bio-techne.com/images/products/nbp3-44027_mouse-ctcf-mab-cl0307-azide-and-bsa-free-282202512182714.jpg "Western Blot: CTCF Antibody [NBP3-44027]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL0307
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P49711, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for CTCF Antibody (CL0307) - Azide and BSA Free
Western Blot: CTCF Antibody [NBP3-44027]
Lane 1: Marker [kDa]Lane 2: Human cell line U-251 MG
![CTCF Antibody (CL0307) - Azide and BSA Free Immunohistochemistry-Paraffin: CTCF Antibody [NBP3-44027]](https://resources.bio-techne.com/images/products/nbp3-44027_mouse-ctcf-mab-cl0307-azide-and-bsa-free-28220251235170.jpg "Immunohistochemistry-Paraffin: CTCF Antibody [NBP3-44027]")
Immunohistochemistry-Paraffin: CTCF Antibody [NBP3-44027]
Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.![CTCF Antibody (CL0307) - Azide and BSA Free Immunohistochemistry-Paraffin: CTCF Antibody [NBP3-44027]](https://resources.bio-techne.com/images/products/nbp3-44027_mouse-ctcf-mab-cl0307-azide-and-bsa-free-28220251233299.jpg "Immunohistochemistry-Paraffin: CTCF Antibody [NBP3-44027]")
Immunohistochemistry-Paraffin: CTCF Antibody [NBP3-44027]
Staining of human kidney shows strong nuclear positivity in cells in glomeruli and tubuli.![CTCF Antibody (CL0307) - Azide and BSA Free Immunohistochemistry-Paraffin: CTCF Antibody [NBP3-44027]](https://resources.bio-techne.com/images/products/nbp3-44027_mouse-ctcf-mab-cl0307-azide-and-bsa-free-28220251324556.jpg "Immunohistochemistry-Paraffin: CTCF Antibody [NBP3-44027]")
![CTCF Antibody (CL0307) - Azide and BSA Free Immunohistochemistry-Paraffin: CTCF Antibody [NBP3-44027]](https://resources.bio-techne.com/images/products/nbp3-44027_mouse-ctcf-mab-cl0307-azide-and-bsa-free-282202513254711.jpg "Immunohistochemistry-Paraffin: CTCF Antibody [NBP3-44027]")
Applications for CTCF Antibody (CL0307) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized in 5% Trehalose.
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CTCF
Long Name
CCCTC-binding Factor
Alternate Names
11-Zinc Finger Protein2, CCCTC-binding Factor
Gene Symbol
CTCF
Additional CTCF Products
Product Documents for CTCF Antibody (CL0307) - Azide and BSA Free
Product Specific Notices for CTCF Antibody (CL0307) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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