CTGF/CCN2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66426
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Applications
Control, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Summary for CTGF/CCN2 Antibody
Immunogen
Peptide with sequence C-SLYYRKMYGDMA, from the C Terminus of the protein sequence according to NP_001892.
Reactivity Notes
Expected from sequence similarity: Human, Rat and Canine
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for CTGF/CCN2 Antibody
![Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NBP2-66426]](https://resources.bio-techne.com/images/products/CTGF-CCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-66426-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NBP2-66426]")
Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NBP2-66426]
Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NBP2-66426] - Paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).![Immunohistochemistry: CTGF/CCN2 Antibody [NBP2-66426]](https://resources.bio-techne.com/images/products/CTGF-CCN2-Antibody-Immunohistochemistry-NBP2-66426-img0003.jpg "Immunohistochemistry: CTGF/CCN2 Antibody [NBP2-66426]")
Immunohistochemistry: CTGF/CCN2 Antibody [NBP2-66426]
Immunohistochemistry: CTGF/CCN2 Antibody [NBP2-66426] - (6ug/ml) staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Control: CTGF/CCN2 Antibody [NBP2-66426] - Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.
Applications for CTGF/CCN2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5 ug/mL
Immunohistochemistry
6.0 - 8.0 ug/ml
Peptide ELISA
Detection limit 1:10000
Application Notes
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CTGF/CCN2
CTGF antibodies are useful tools for angiogenesis and cell structure research, and for studies on certian types of cancer.
Long Name
Connective Tissue Growth Factor
Alternate Names
CCN2, CTGRP, Fisp12, HCS24, IGFBP-8, NOV2
Gene Symbol
CCN2
Additional CTGF/CCN2 Products
Product Specific Notices for CTGF/CCN2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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