CtIP Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-79810
![Western Blot: CtIP Antibody [NB100-79810] Western Blot: CtIP Antibody [NB100-79810]](https://resources.bio-techne.com/images/products/CtIP-Antibody-Western-Blot-NB100-79810-img0006.jpg)
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 850 and the C-terminus (residue 897) of human CtBP Interacting Protein (Retinoblastoma Binding Protein 8) using the numbering given in entry NP_002885.1 (GeneID 5932).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CtIP Antibody - BSA Free
Western Blot: CtIP Antibody [NB100-79810]
Western Blot: CtIP Antibody [NB100-79810] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-CtIP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 minutes.Immunoprecipitation: CtIP Antibody [NB100-79810]
Immunoprecipitation: CtIP Antibody [NB100-79810] - Detection of human CtIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CtIP antibody NB100-79810 used for IP at 3 ug per reaction. CtIP was also immunoprecipitated by rabbit anti-CtIP antibody NB100-79809. For blotting immunoprecipitated CtIP, NB100-79810 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for CtIP Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 26215093).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CtIP
Alternate Names
CtBP-interacting protein, CtIP, DNA endonuclease RBBP8, EC 3.1, RBBP-8, retinoblastoma binding protein 8, Retinoblastoma-binding protein 8, Retinoblastoma-interacting protein and myosin-like, RIMSAE2, Sporulation in the absence of SPO11 protein 2 homolog
Entrez Gene IDs
5932 (Human)
Gene Symbol
RBBP8
UniProt
Additional CtIP Products
Product Documents for CtIP Antibody - BSA Free
Product Specific Notices for CtIP Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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