CTU2 Antibody (PSH04-05)

Western Blot: CTU2 Antibody (PSH04-05) [NBP3-32231]

Western Blot: CTU2 Antibody (PSH04-05) [NBP3-32231] - Western blot analysis of CTU2 on different lysates with Rabbit anti-CTU2 antibody (NBP3-32231) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HT-29 cell lysate
Lane 3: 293T cell lysate
Lane 4: K-562 cell lysate
Lane 5: Raji cell lysate
Lane 6: HepG2 cell lysate
Lane 7: COS-1 cell lysate
Lane 8: NIH/3T3 cell lysate
Lane 9: Mouse spleen tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 56 kDa
Observed band size: 54/56 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32231) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CTU2 Antibody (PSH04-05) [NBP3-32231]

Immunohistochemistry: CTU2 Antibody (PSH04-05) [NBP3-32231] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CTU2 antibody (NBP3-32231) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32231) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.