Cullin 5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-22970

Novus Biologicals, part of Bio-Techne
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NBP1-22970
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Orangutan (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Cullin 5 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human cullin-5 using the numbering given in entry NP_003469.2 (GeneID 8065).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cullin 5 Antibody

Western Blot: Cullin 5 Antibody [NBP1-22970]

Western Blot: Cullin 5 Antibody [NBP1-22970]

Western Blot: Cullin 5 Antibody [NBP1-22970] - Whole cell lysate (10 ug) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cul5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: Cullin 5 Antibody [NBP1-22970]

Immunohistochemistry-Paraffin: Cullin 5 Antibody [NBP1-22970]

Immunohistochemistry-Paraffin: Cullin 5 Antibody [NBP1-22970] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Cul5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: Cullin 5 Antibody [NBP1-22970]

Western Blot: Cullin 5 Antibody [NBP1-22970]

Western Blot: Cullin 5 Antibody [NBP1-22970] - by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. Antibodies: Affinity purified rabbit anti-Cul5 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. Cul5 was also immunoprecipitated by another rabbit anti-Cul5 antibody, which recognizes a downstream epitope.
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Applications for Cullin 5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500- 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cullin 5

Cullin-5 (Cul-5) is a core component of multiple E3 ubiquitin ligase complexes.

Alternate Names

CUL-5, cullin 5, VACM-1cullin-5, VACM1Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1), Vasopressin-activated calcium-mobilizing receptor 1, Vasopressin-activated calcium-mobilizing receptor-1

Entrez Gene IDs

8065 (Human)

Gene Symbol

CUL5

UniProt

NP_003469.2 (Human)

Additional Cullin 5 Products

Product Documents for Cullin 5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cullin 5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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