Custos Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91713

Novus Biologicals, part of Bio-Techne
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NBP1-91713
NBP1-91713-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Custos Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AWGLEQRPHVAGKPRAGAANSQLSTSQPSLRHKVNEHEQDGNELQTTPEFRAHVAKKLGALLDSFITISEAAKEPAKAKVQKVALEDDGFRLFFTSVPGGREKEESPQPR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Custos Antibody

Western Blot: Custos Antibody [NBP1-91713]

Western Blot: Custos Antibody [NBP1-91713]

Western Blot: C12orf43 Antibody [NBP1-91713] - Analysis in control (vector only transfected HEK293T lysate) and C12orf43 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: Custos Antibody [NBP1-91713]

Immunocytochemistry/ Immunofluorescence: Custos Antibody [NBP1-91713]

Immunocytochemistry/Immunofluorescence: C12orf43 Antibody [NBP1-91713] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.
Immunohistochemistry-Paraffin: Custos Antibody [NBP1-91713]

Immunohistochemistry-Paraffin: Custos Antibody [NBP1-91713]

Immunohistochemistry-Paraffin: C12orf43 Antibody [NBP1-91713] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Applications for Custos Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Custos

Alternate Names

chromosome 12 open reading frame 43, FLJ12448, hypothetical protein LOC64897

Gene Symbol

C12ORF43

Additional Custos Products

Product Documents for Custos Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Custos Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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