Key Product Details

Species Reactivity

Mouse

Applications

ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Neutralization, Western Blot

Label

Janelia Fluor 646

Antibody Source

Recombinant Monoclonal Rat IgG2A Clone # 126315

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CX3CL1/Fractalkine Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CX3CL1/Fractalkine, S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CX3CL1/Fractalkine, and E. coli-derived recombinant mouse CX3CL1/Fractalkine

Specificity

Detects mouse CX3CL1/Fractalkine in Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with the chemokine domain of recombinant human (rh) CX3CL1, 13% cross-reactivity with rhCX3CL1, and 50% cross-reactivity with recombinant rat CX3CL1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for CX3CL1/Fractalkine Antibody (126315) [Janelia Fluor® 646]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CX3CL1/Fractalkine

CX3CL1, also known as fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, neurotactin, small-inducible cytokine D1, FKN, NTT, and SCYD1, is a member of the intercrine delta family. The soluble form of CX3CL1 is chemotactic for monocytes and T-cells, but not for neutrophils. CX3CL1's membrane bound form promotes adhesion of the monocytes and T-cells to endothelial cells. Additionally, CX3CL1 binds to CX3CR1 and regulates adhesion and migration of leukocyte processes at the endothelium.

Alternate Names

FKN, Fractalkine, Neurotactin

Gene Symbol

CX3CL1

Additional CX3CL1/Fractalkine Products

Product Documents for CX3CL1/Fractalkine Antibody (126315) [Janelia Fluor® 646]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CX3CL1/Fractalkine Antibody (126315) [Janelia Fluor® 646]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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