Key Product Details

Species Reactivity

Human, Primate

Applications

CyTOF-ready, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Intracellular Staining by Flow Cytometry, Neutralization, Western Blot

Label

DyLight 594 (Excitation = 593 nm, Emission = 618 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 20326

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CXCL1/GRO alpha/KC/CINC-1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CXCL1/GRO alpha/KC/CINC-1

Specificity

Detects human CXCL1/GRO alpha/KC/CINC-1 in ELISAs and Western blots. In Western blots, this antibody shows approximately 20% cross-reactivity with recombinant human (rh) CXCL2/GRO beta and rhCXCL3/GRO gamma and no cross-reactivity with recombinant rat (rr)CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhMIP-1 alpha, recombinant mouse (rm)MIP-1 alpha, rmMIP-1 beta, rmMIP-1 beta, rhMIP-1 delta, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, or rmMIP-3 beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for CXCL1/GRO alpha/KC/CINC-1 Antibody (20326) [DyLight 594]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha

Gene Symbol

CXCL1

Additional CXCL1/GRO alpha/KC/CINC-1 Products

Product Documents for CXCL1/GRO alpha/KC/CINC-1 Antibody (20326) [DyLight 594]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CXCL1/GRO alpha/KC/CINC-1 Antibody (20326) [DyLight 594]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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