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CXCL10/IP-10/CRG-2 Antibody (JA10-82)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67004

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67004

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JA10-82

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide within Human CXCL10/IP-10/CRG-2 aa 49-98 / 98. (SwissProt: P02778 Human)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CXCL10/IP-10/CRG-2 Antibody (JA10-82)

Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]

Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]

Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]

Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]

Immunohistochemistry-Paraffin: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Human tonsil tissue using anti-IP10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]

Immunocytochemistry/ Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004]

Immunocytochemistry/Immunofluorescence: CXCL10/IP-10/CRG-2 Antibody (JA10-82) [NBP2-67004] - Staining IP10 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications for CXCL10/IP-10/CRG-2 Antibody (JA10-82)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CXCL10/IP-10/CRG-2

IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.

Alternate Names

CRG-2, CRG2, IP-10

Gene Symbol

CXCL10

Additional CXCL10/IP-10/CRG-2 Products

Product Documents for CXCL10/IP-10/CRG-2 Antibody (JA10-82)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CXCL10/IP-10/CRG-2 Antibody (JA10-82)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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