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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10C6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Full length recombinant protein expressed in E-Coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CXCL11/I-TAC Antibody (10C6)

Western Blot: CXCL11/I-TAC Antibody (10C6) [NBP2-22533]

Western Blot: CXCL11/I-TAC Antibody (10C6) [NBP2-22533]

Western Blot: CXCL11/I-TAC Antibody (10C6) [NBP2-22533] - Analysis of 0.25 ug of recombinant human ITAC and induced HeLa cell culture supernatant onto a 4-20% Tris-HCl polyacrylamide gel.
ELISA: CXCL11/I-TAC Antibody (10C6) [NBP2-22533]

ELISA: CXCL11/I-TAC Antibody (10C6) [NBP2-22533]

ELISA: CXCL11/I-TAC Antibody (10C6) [NBP2-22533] - Human ITAC was performed by coating a blank 96-well microtiter plate with 100ul per well of Human ITAC monoclonal antibody in duplicate at 1, 3, 4, 5, 7, and 9ug/ml in DPBS and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300ul per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human ITAC recombinant protein at 50ul per well was added in duplicate at 4000, 1600, 640, 256, 102.4, 40.96, and 0 pg/ml for 2 hours at room temperature along with 50ul of Human ITAC biotinylated monoclonal antibody in all applicable wells at 0.2ug/ml for 2 hours at room temperature. The plate was washed with ELISA Wash Buffer and incubated with 100ul per well of Streptavidin-HRP in all test wells at 1:80,000 dilution for 1 hour at room temperature and then washed and incubated with 100ul per well of TMB substrate for 30 minutes at room temperature in the dark.

Applications for CXCL11/I-TAC Antibody (10C6)

Application
Recommended Usage

ELISA

1 - 3 ug/ml

Western Blot

1:1000
Application Notes
Sandwich ELISA: 1 ug/ml for coating and 0.125 - 0.25 ug/ml for detection. The Molecular weight is ~8303 Da from positions 22 - 94 as determined by MALDI.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CXCL11/I-TAC

Small inducible cytokine B11 precursor; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; small inducible cytokine subfamily B (Cys-X-Cys), member 11. This protein is a CXC member of the chemokine superfamily. It induces a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene.

Alternate Names

beta-R1, H174, I-TAC, ITAC, SCYB9B

Gene Symbol

CXCL11

UniProt

Additional CXCL11/I-TAC Products

Product Documents for CXCL11/I-TAC Antibody (10C6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CXCL11/I-TAC Antibody (10C6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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