CXCL13/BLC/BCA-1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16041
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Rhesus Macaque (93%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
0.74 mg/ml
Product Specifications
Immunogen
Synthetic peptide made to a C-terminal region of human CXCL13 (between amino acids 50-109) [UniProt O43927]
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CXCL13/BLC/BCA-1 Antibody - BSA Free
Western Blot: CXCL13/BLC/BCA-1 AntibodyBSA Free [NBP2-16041]
Western Blot: CXCL13/BLC/BCA-1 Antibody [NBP2-16041] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: CXCL13/BLC/BCA-1 Antibody - BSA Free [NBP2-16041]
Immunocytochemistry/Immunofluorescence: CXCL13/BLC/BCA-1 Antibody [NBP2-16041] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-CXCL13 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Immunohistochemistry-Paraffin: CXCL13/BLC/BCA-1 Antibody - BSA Free [NBP2-16041]
Immunohistochemistry-Paraffin: CXCL13/BLC/BCA-1 Antibody - BSA Free [NBP2-16041] - Human breast cancer tissue stained for CXCL13/BLC/BCA-1 (green) and counterstained with DAPI (blue). Alexa Fluor 488 version of antibody used (NBP2-16041AF488). Image from verified customer review.Applications for CXCL13/BLC/BCA-1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5 ug/mL
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Western Blot
1:500 - 1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
0.74 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CXCL13/BLC/BCA-1
Additional CXCL13/BLC/BCA-1 Products
Product Documents for CXCL13/BLC/BCA-1 Antibody - BSA Free
Product Specific Notices for CXCL13/BLC/BCA-1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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