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CXCL13/BLC/BCA-1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16041

Novus Biologicals, part of Bio-Techne
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NBP2-16041
NBP2-16041SS

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rhesus Macaque (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.74 mg/ml

Product Specifications

Immunogen

Synthetic peptide made to a C-terminal region of human CXCL13 (between amino acids 50-109) [UniProt O43927]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CXCL13/BLC/BCA-1 Antibody - BSA Free

Western Blot: CXCL13/BLC/BCA-1 AntibodyBSA Free [NBP2-16041]

Western Blot: CXCL13/BLC/BCA-1 AntibodyBSA Free [NBP2-16041]

Western Blot: CXCL13/BLC/BCA-1 Antibody [NBP2-16041] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: CXCL13/BLC/BCA-1 Antibody - BSA Free [NBP2-16041]

Immunocytochemistry/ Immunofluorescence: CXCL13/BLC/BCA-1 Antibody - BSA Free [NBP2-16041]

Immunocytochemistry/Immunofluorescence: CXCL13/BLC/BCA-1 Antibody [NBP2-16041] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-CXCL13 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: CXCL13/BLC/BCA-1 Antibody - BSA Free [NBP2-16041]

Immunohistochemistry-Paraffin: CXCL13/BLC/BCA-1 Antibody - BSA Free [NBP2-16041]

Immunohistochemistry-Paraffin: CXCL13/BLC/BCA-1 Antibody - BSA Free [NBP2-16041] - Human breast cancer tissue stained for CXCL13/BLC/BCA-1 (green) and counterstained with DAPI (blue). Alexa Fluor 488 version of antibody used (NBP2-16041AF488). Image from verified customer review.

Applications for CXCL13/BLC/BCA-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/mL

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1:500 - 1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

0.74 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CXCL13/BLC/BCA-1

B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq]

Alternate Names

ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13

Gene Symbol

CXCL13

UniProt

Additional CXCL13/BLC/BCA-1 Products

Product Documents for CXCL13/BLC/BCA-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CXCL13/BLC/BCA-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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