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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4.4

Format

BSA Free

Concentration

1 mg/ml

Product Summary for CXCL16 Antibody (4.4) - BSA Free

Immunogen

GST-CXCL16 (143-224aa) fusion protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CXCL16 Antibody (4.4) - BSA Free

Western Blot: CXCL16 Antibody (4.4)BSA Free [NBP2-50187]

Western Blot: CXCL16 Antibody (4.4)BSA Free [NBP2-50187]

Western Blot: CXCL16 Antibody (4.4) [NBP2-50187] - Analysis of lysates prepared from HEK293 FT cells with a plasmid expressing the full length CXCL16 open reading frame. Lane 1: 293FT transfected cell lysate probed with anti-CXCL16 [4.4], Lane 2: 293FT mock cell lysate probed with the same antibody.
Immunocytochemistry/ Immunofluorescence: CXCL16 Antibody (4.4) - BSA Free [NBP2-50187]

Immunocytochemistry/ Immunofluorescence: CXCL16 Antibody (4.4) - BSA Free [NBP2-50187]

Immunocytochemistry/Immunofluorescence: CXCL16 Antibody (4.4) [NBP2-50187] - Staining of cos7 cells transfected with a plasmid containing a full-length CXCL16 open reading frame (a). 48 hours post transfection cells were fixed with 4% paraformaldehyde permeabilised with 0.1% Triton X-100 and stained using Anti-CXCL16 [4.4] and followed by secondary goat anti-mouse FITC (1:100). Nucleic acid DAPI counterstain is shown in blue. A negative control mock is also shown (b).

Applications for CXCL16 Antibody (4.4) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:100 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CXCL16

CXC chemokine ligand 16 (CXCL16) is a type I membrane protein that has a non-ELR CXC chemokine domain in its extracellular region. Fractalkine (CX3CL1) and CXCL16 constitute the only two transmembrane chemokines. Mouse and human CXCL16 share 70% amino acid sequence similarity within their chemokine domains and 49% overall amino acid sequence identity.

Alternate Names

CXCL16

Gene Symbol

CXCL16

Additional CXCL16 Products

Product Documents for CXCL16 Antibody (4.4) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CXCL16 Antibody (4.4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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