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CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43339

Novus Biologicals, part of Bio-Techne
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NBP1-43339-0.025mg
NBP1-43339-0.1mg
Conjugate
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Key Product Details

Species Reactivity

Validated:

Human, Hamster

Applications

Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 8F1-1-4

Format

BSA Free

Concentration

0.5 mg/ml

Product Summary for CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free

Immunogen

The immunogen for this antibody was CD181.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free

Fluorophore-linked immunosorbent assay: CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free [NBP1-43339]

Fluorophore-linked immunosorbent assay: CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free [NBP1-43339]

Fluorophore-linked immunosorbent assay: CXCR1/IL-8 RA Antibody (8F1-1-4) [NBP1-43339] - Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG2b kappa Isotype Control Purified (left) or 0.25 ug of Anti-Human CD181 (CXCR1) Purified (right) followed by F(ab')2 Anti-Mouse IgG PE.

Applications for CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Fluorophore-linked immunosorbent assay

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CXCR1/IL-8RA

The 8F1-1-4 monoclonal antibody reacts with human CD181 (CXCR1, IL-8Ralpha). CD181 is a 67-70 kDa member of the 7-transmembrane spanning G-protein coupled receptor (GPCR) family. CD181 is expressed as a homodimer, or a heterodimer with CD182 (CXCR2, IL-8Rbeta) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of its ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.

Long Name

Interleukin 8 Receptor A

Alternate Names

CD181, IL-8 RA, IL8RA

Entrez Gene IDs

3577 (Human)

Gene Symbol

CXCR1

UniProt

Additional CXCR1/IL-8RA Products

Product Documents for CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CXCR1/IL-8RA Antibody (8F1-1-4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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