CXCR3 Antibody (3B7Y7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15369

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15369-100ul
NBP3-15369-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3B7Y7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CXCR3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human CXCR3 (P49682). ATHCQYNFPQVGRTALRVLQLVAGFLLPLLVMAYCYAHILAVLLVSRGQRRLRAMRLVVVVVVAFALCWTPYHLVVLVDILMDLGALARNCGRESRVDVAK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CXCR3 Antibody (3B7Y7)

Western Blot: CXCR3 Antibody (3B7Y7) [NBP3-15369]

Western Blot: CXCR3 Antibody (3B7Y7) [NBP3-15369]

Western Blot: CXCR3 Antibody (3B7Y7) [NBP3-15369] - Western blot analysis of extracts of K-562 cells, using CXCR3 Rabbit mAb (NBP3-15369) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry: CXCR3 Antibody (3B7Y7) [NBP3-15369]

Immunohistochemistry: CXCR3 Antibody (3B7Y7) [NBP3-15369]

Immunohistochemistry: CXCR3 Antibody (3B7Y7) [NBP3-15369] - Immunofluorescence analysis of mouse spleen using CXCR3 Rabbit mAb (NBP3-15369) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for CXCR3 Antibody (3B7Y7)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CXCR3

CXCR3, also known as GPR9, is a member of the CXC chemokine receptor subfamily. Similar to other chemokine receptors, it is a G protein-coupled receptor (GPCR). In humans, two alternatively spliced CXCR3 isoforms, CXCR3 and CXCR3B, have been identified and have different chemokine binding affinities. Human CXCR3 is 368 amino acids (aa) in length with a predicted molecular weight of 41 kDa. CXCR3B contains an additional N-terminal sequence and is 415 aa in length with a predicted molecular weight of 46 kDa. Mouse and rat CXCR3 share 86% aa sequence identity with human CXCR3. CXCR3 is expressed on leukocytes and is upregulated following T cell activation. It binds multiple CXC chemokines and is known to mediate leukocyte trafficking. CXCR3 has been implicated in neuroinflammatory and autoimmune diseases.

Alternate Names

CD183, CXCR3, GPR9

Gene Symbol

CXCR3

Additional CXCR3 Products

Product Documents for CXCR3 Antibody (3B7Y7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CXCR3 Antibody (3B7Y7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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