CXCR3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56404

Novus Biologicals, part of Bio-Techne
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NB100-56404
NB100-56404SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CXCR3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids 290-303 of human CXCR3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CXCR3 Antibody

Western Blot: CXCR3 Antibody [NB100-56404]

Western Blot: CXCR3 Antibody [NB100-56404]

Western Blot: CXCR3 Antibody [NB100-56404] - HEK293T cells. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: CXCR3 Antibody [NB100-56404]

Immunocytochemistry/ Immunofluorescence: CXCR3 Antibody [NB100-56404]

Immunocytochemistry/Immunofluorescence: CXCR3 Antibody [NB100-56404] - Brain microvascular endothelial cells isolated from WT and CXCR3 KO mice were stained with anti-CXCR3 (green) and anti-ZO-1 (blue) antibodies to analyze CXCR3 expression by immunofluorescence. Image from verified customer review.
Immunohistochemistry-Paraffin: CXCR3 Antibody [NB100-56404]

Immunohistochemistry-Paraffin: CXCR3 Antibody [NB100-56404]

Immunohistochemistry-Paraffin: CXCR3 Antibody [NB100-56404] - IHC analysis of a FFPE human spleen using 1:25 dilution of CXCR3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of HIER using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (40X) was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
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Applications for CXCR3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 31887215)

Immunohistochemistry-Frozen

reported in scientific literature (PMID 19234157)

Immunohistochemistry-Paraffin

1:10 - 1:500

Proximity Ligation Assay

reported in scientific literature (PMID 34433033)

Western Blot

1 - 3 ug/mL
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NB100-56404 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CXCR3

Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Historically, CD183 was the third CXC chemokine receptor discovered and therefore, commonly designated as CXCR3. Binding of chemokines to CD183 induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. The CXC chemokine receptor (CXCR) 3, a G-protein-coupled 7-transmembrane receptor, is expressed at high levels on activated and memory T cells, B cells, natural killer cells, and plasmacytoid monocytes.

Alternate Names

CD183, CXCR3, GPR9

Entrez Gene IDs

2833 (Human); 12766 (Mouse)

Gene Symbol

CXCR3

UniProt

P49682

Additional CXCR3 Products

Product Documents for CXCR3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CXCR3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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