CXXC1 Antibody (PSH01-54)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32183
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH01-54
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human CXXC1 aa 300-656. (Uniprot: Q9P0U4)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CXXC1 Antibody (PSH01-54)
Western Blot: CXXC1 Antibody (PSH01-54) [NBP3-32183]
Western Blot: CXXC1 Antibody (PSH01-54) [NBP3-32183] - Western blot analysis of CXXC1 on different lysates with Rabbit anti-CXXC1 antibody (NBP3-32183) at 1/1,000 dilution.Lane 1: HepG2 cell lysate (20 ug/Lane)
Lane 2: NIH/3T3 cell lysate (20 ug/Lane)
Lane 3: K-562 cell lysate (20 ug/Lane)
Lane 4: MCF7 cell lysate (20 ug/Lane)
Lane 5: RAW264.7 cell lysate (20 ug/Lane)
Lane 6: Mouse brain tissue lysate (40 ug/Lane)
Lane 7: L6 cell lysate (20 ug/Lane)
Lane 8: PC-12 cell lysate (20 ug/Lane)
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 5 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32183) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: CXXC1 Antibody (PSH01-54) [NBP3-32183]
Immunohistochemistry: CXXC1 Antibody (PSH01-54) [NBP3-32183] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-CXXC1 antibody (NBP3-32183) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32183) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for CXXC1 Antibody (PSH01-54)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CXXC1
Alternate Names
CFP1CXXC-type zinc finger protein 1, CGBPPHD finger and CXXC domain-containing protein 1, CpG binding protein, cpG-binding protein, CXXC finger 1 (PHD domain), CXXC finger protein 1, DNA-binding protein with PHD finger and CXXC domain, hCGBP, HsT2645, PCCX12410002I16Rik, PHF185830420C16Rik, SPP1, ZCGPC1, zinc finger, CpG binding-type containing 1
Gene Symbol
CXXC1
Additional CXXC1 Products
Product Documents for CXXC1 Antibody (PSH01-54)
Product Specific Notices for CXXC1 Antibody (PSH01-54)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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