Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all CXXC4 Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CXXC4. The immunogen is located within amino acids 50 - 100 of CXXC4. Amino Acid Squence: NLPERVGTFSAIPALGG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CXXC4 Antibody - BSA Free

Western Blot: CXXC4 AntibodyBSA Free [NBP1-76491]

Western Blot: CXXC4 AntibodyBSA Free [NBP1-76491]

Western Blot: CXXC4 Antibody [NBP1-76491] - Human brain tissue lysate with CXXC4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
CXXC4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CXXC4 Antibody - BSA Free [NBP1-76491] -

Immunocytochemistry/ Immunofluorescence: CXXC4 Antibody - BSA Free [NBP1-76491] - Immunofluorescence of CXXC4 in human brain tissue with CXXC4 antibody at 20 u/mL.
CXXC4 Antibody - BSA Free

Immunohistochemistry: CXXC4 Antibody - BSA Free [NBP1-76491] -

Immunohistochemistry: CXXC4 Antibody - BSA Free [NBP1-76491] - Immunohistochemistry of CXXC4 in human brain tissue with CXXC4 antibody at 10 u/mL.

Applications for CXXC4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CXXC4

CXXC4 is zinc finger protein that binds directly to the PDZ domain of DVL1, a protein involved in the Wnt signaling pathway, through its C-terminal region. CXXC4's interaction with DVL1 competes with DVL1-AXIN binding, although the affinity of DVL1 for CXXC4 is lower than that for AXIN. In mouse fibroblasts, CXXC4 suppressed Wnt3a induction of beta-catenin and inhibited Wnt3a-dependent TCF4 activation, suggesting that CXXC4 acts as a negative regulator of the Wnt signaling pathway and functions between DVL and beta-catenin. Recent reports suggest that decreased CXXC4 expression is associated with kidney cancer metastasis, cell proliferation, reduced apoptosis in response to cancer drugs, and upregulation of genes involved with proliferation, invasion and cell survival, suggesting that CXXC4 plays a critical role in tumor progression in kidney cancer.

Alternate Names

CXXC finger protein 4, CXXC-type zinc finger protein 4, Dvl-binding protein IDAX (inhibition of the Dvl and Axin complex), IDAXCXXC finger 4, Inhibition of the Dvl and axin complex protein, MGC149872

Gene Symbol

CXXC4

UniProt

NP_079488 (Human)

Additional CXXC4 Products

Product Documents for CXXC4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CXXC4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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