CYB5R3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84252

Novus Biologicals, part of Bio-Techne
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NBP1-84252-25ul
NBP1-84252
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CYB5R3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIMKDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEAWDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDH

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%). Mouse reactivity reported in scientific literature (PMID: 25144769).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CYB5R3 Antibody

Western Blot: CYB5R3 Antibody [NBP1-84252]

Western Blot: CYB5R3 Antibody [NBP1-84252]

Western Blot: CYB5R3 Antibody [NBP1-84252] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: CYB5R3 Antibody [NBP1-84252]

Immunocytochemistry/ Immunofluorescence: CYB5R3 Antibody [NBP1-84252]

Immunocytochemistry/Immunofluorescence: CYB5R3 Antibody [NBP1-84252] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CYB5R3 Antibody [NBP1-84252]

Immunohistochemistry-Paraffin: CYB5R3 Antibody [NBP1-84252]

Immunohistochemistry-Paraffin: CYB5R3 Antibody [NBP1-84252] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.

Applications for CYB5R3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 25144769). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYB5R3

Alternate Names

B5RNADH-cytochrome b5 reductase 3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1NADH-cytochrome b5 reductase 3 membrane-bound form, diaphorase (NADH) (cytochrome b-5 reductase), diaphorase-1, EC 1.6.2.2, NADH-cytochrome b5 reductase 3 soluble form

Gene Symbol

CYB5R3

Additional CYB5R3 Products

Product Documents for CYB5R3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CYB5R3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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