CYB5R3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06497
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human CYB5R3 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for CYB5R3 Antibody
Western Blot: CYB5R3 Antibody [NBP3-06497]
Western Blot: CYB5R3 Antibody [NBP3-06497] - Lane A: A431 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: U87MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDaImmunohistochemistry-Paraffin: CYB5R3 Antibody [NBP3-06497]
Immunohistochemistry-Paraffin: CYB5R3 Antibody [NBP3-06497] - Staining of human CYB5R3 in human testis with rabbit polyclonal antibody at 1:10000 dilution.Immunoprecipitation: CYB5R3 Antibody [NBP3-06497]
Immunoprecipitation: CYB5R3 Antibody [NBP3-06497] - Lane A:0.5 mg A431 HepG2 HeLa U87MG Whole Cell Lysate4 uL anti-CYB5R3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CYB5R3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :34 kDaApplications for CYB5R3 Antibody
Application
Recommended Usage
Immunohistochemistry
1:1000-1:10000
Immunohistochemistry-Paraffin
1:1000-1:10000
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CYB5R3
Alternate Names
B5RNADH-cytochrome b5 reductase 3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1NADH-cytochrome b5 reductase 3 membrane-bound form, diaphorase (NADH) (cytochrome b-5 reductase), diaphorase-1, EC 1.6.2.2, NADH-cytochrome b5 reductase 3 soluble form
Gene Symbol
CYB5R3
Additional CYB5R3 Products
Product Specific Notices for CYB5R3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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