CYBB/NOX2 Antibody (NL7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41012
![Flow (Intracellular): CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]](https://resources.bio-techne.com/images/products/CYBB-NOX2-Antibody-NL7-Flow-Intracellular-NBP1-41012-img0005.jpg "Flow (Intracellular): CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]")
![Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]](https://resources.bio-techne.com/images/products/CYBB-NOX2-Antibody-NL7-Flow-Cytometry-NBP1-41012-img0004.jpg "Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # NL7
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Cytochrome B245 solubilized in octyl-beta-glucopyranoside
Epitope
Clone NL7 binds amino acids 498-506 (EKDVITGLK), a cytosolic epitope on gp91phox not accessible on intact neutrophils.
Specificity
This antibody recognises the heavy chain of cytochrome B245. It recognises the native as well as the denatured form of the protein
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CYBB/NOX2 Antibody (NL7)
Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]
Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Daudi cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.![Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]](https://resources.bio-techne.com/images/products/CYBB-NOX2-Antibody-NL7-Flow-Cytometry-NBP1-41012-img0001.jpg "Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]")
Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]
Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain. Permeabilised with Leucoperm![Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]](https://resources.bio-techne.com/images/products/CYBB-NOX2-Antibody-NL7-Flow-Cytometry-NBP1-41012-img0003.jpg "Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]")
Flow Cytometry: CYBB/NOX2 Antibody (NL7) - BSA Free [NBP1-41012]
Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Analysis using the PE conjugate of NBP1-41012. Staining of CYBB in human peripheral blood using anti-CYBB antibody. Image from verified customer review.Applications for CYBB/NOX2 Antibody (NL7)
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul (Burrit, J. B. et al.). Western blot detects a broad band at ~90 kDa. Clone NL7 has an inhibitory effect on NADPH oxidase activation. The antibody does not interfere with the translocation of the cytosolic subunits of NADPH oxidase to the cell membrane.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using NBP1-41012 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CYBB/NOX2
Long Name
Cytochrome b-245 heavy chain
Alternate Names
CGD91-phox, CYBB, EC 1.-.-.-, gp91-1, gp91-phox, NADPH oxidase 2, NOX2
Entrez Gene IDs
1536 (Human)
Gene Symbol
CYBB
UniProt
Additional CYBB/NOX2 Products
Product Documents for CYBB/NOX2 Antibody (NL7)
Product Specific Notices for CYBB/NOX2 Antibody (NL7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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