CYBB/NOX2 Antibody (SN07-16)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67680
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SN07-16
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human CYBB/NOX2 aa 131-180 / 570. (SwissProt: P04839 Human; SwissProt: Q61093 Mouse; Entrez Gene:66021 Rat)
Localization
Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CYBB/NOX2 Antibody (SN07-16)
Western Blot: CYBB/NOX2 Antibody (SN07-16) [NBP2-67680] -
Western Blot: CYBB/NOX2 Antibody (SN07-16) [NBP2-67680] Analysis of NOX2/gp91phox on different lysates with Rabbit anti-NOX2/gp91phox antibody at 1/2,000 dilution.Lane 1: HepG2 cell lysate (15 ug/Lane)Lane 2: MCF7 cell lysate (15 ug/Lane)Lane 3: Mouse brain tissue lysate (20 ug/Lane)Lane 4: Mouse spleen tissue lysate (20 ug/Lane)Lane 5: Rat brain tissue lysate (20 ug/Lane)Lane 6: Rat spleen tissue lysate (20 ug/Lane)Predicted band size: 65 kDaObserved band size: 55 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.Applications for CYBB/NOX2 Antibody (SN07-16)
Application
Recommended Usage
Western Blot
1:2,000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CYBB/NOX2
Long Name
Cytochrome b-245 heavy chain
Alternate Names
CGD91-phox, CYBB, EC 1.-.-.-, gp91-1, gp91-phox, NADPH oxidase 2, NOX2
Gene Symbol
CYBB
Additional CYBB/NOX2 Products
Product Documents for CYBB/NOX2 Antibody (SN07-16)
Product Specific Notices for CYBB/NOX2 Antibody (SN07-16)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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