Cyclin A2 Antibody (3E9Z3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15625
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3E9Z3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 11-210 of human Cyclin A2 (P20248). TREAGSALLALQQTALQEDQENINPEKAAPVQQPRTRAALAVLKSGNPRGLAQQQRPKTRRVAPLKDLPVNDEHVTVPPWKANSKQPAFTIHVDEAEKEAQKKPAESQKIEREDALAFNSAISLPGPRKPLVPLDYPMDGSFESPHTMDMSIILEDEKPVSVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSM
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cyclin A2 Antibody (3E9Z3)
![Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625]](https://resources.bio-techne.com/images/products/Cyclin-A2-Antibody-3E9Z3-Western-Blot-NBP3-15625-img0007.jpg "Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625]")
Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625]
Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of various cell lines, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625]](https://resources.bio-techne.com/images/products/Cyclin-A2-Antibody-3E9Z3-Western-Blot-NBP3-15625-img0008.jpg "Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625]")
Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625]
Western Blot: Cyclin A2 Antibody (3E9Z3) [NBP3-15625] - Western blot analysis of extracts of Mouse testis, using Cyclin A2 antibody (NBP3-15625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Analysis o.f 300ug extracts of HeLa cells using 3ug Cyclin A2 antibody. Western blot was performed from the immunoprecipitate using Cyclin A2 antibody at a dilution of 1:1000.
Analysis o.f 300ug extracts of HeLa cells using 3ug Cyclin A2 antibody. Western blot was performed from the immunoprecipitate using Cyclin A2 antibody at a dilution of 1:1000.
Applications for Cyclin A2 Antibody (3E9Z3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:100 - 1:500
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cyclin A2
Alternate Names
CCNA, CCNA2
Gene Symbol
CCNA2
Additional Cyclin A2 Products
Product Documents for Cyclin A2 Antibody (3E9Z3)
Product Specific Notices for Cyclin A2 Antibody (3E9Z3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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