Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08789
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free [NBP3-08789]](https://resources.bio-techne.com/images/products/Cyclin-A2-Antibody-CCNA2-2333-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08789-img0001.jpg "Immunohistochemistry-Paraffin: Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free [NBP3-08789]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # CCNA2/2333
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free
Immunogen
Full length recombinant Cyclin A2 protein (Uniprot: P20248)
Epitope
aa 144-148
Localization
Nuclear and Cytoplasmic
Specificity
It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07468).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free
Immunohistochemistry-Paraffin: Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free [NBP3-08789]
Immunohistochemistry-Paraffin: Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free [NBP3-08789] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Cyclin A2 Mouse Monoclonal Antibody (CCNA2/2333).![Immunohistochemistry-Paraffin: Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free [NBP3-08789]](https://resources.bio-techne.com/images/products/Cyclin-A2-Antibody-CCNA2-2333-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08789-img0002.jpg "Immunohistochemistry-Paraffin: Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free [NBP3-08789]")
Immunohistochemistry-Paraffin: Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free [NBP3-08789]
Immunohistochemistry-Paraffin: Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free [NBP3-08789] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Cyclin A2 Mouse Monoclonal Antibody (CCNA2/2333).Applications for Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Cyclin A2
Alternate Names
CCNA, CCNA2
Gene Symbol
CCNA2
Additional Cyclin A2 Products
Product Documents for Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free
Product Specific Notices for Cyclin A2 Antibody (CCNA2/2333) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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