Cyclin A2 Antibody (E67)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34311
![Western Blot: Cyclin A2 Antibody (E67) [NBP2-34311]](https://resources.bio-techne.com/images/products/Cyclin-A2-Antibody-E67-Western-Blot-NBP2-34311-img0003.jpg "Western Blot: Cyclin A2 Antibody (E67) [NBP2-34311]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine, Mink, Monkey
Cited:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # E67
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Full length bovine Cyclin A2 protein (Uniprot: P20248)
Epitope
aa 144-148
Reactivity Notes
African Green Monkey, Mink (Mustela)
Localization
Predominantly nuclear
Specificity
It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34613)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cyclin A2 Antibody (E67)
Western Blot: Cyclin A2 Antibody (E67) [NBP2-34311]
Western Blot: Cyclin A2 Antibody (E67) [NBP2-34311] - Western Blot Analysis of HeLa cell lysate using Cyclin A2 Antibody (E67).![Immunohistochemistry-Paraffin: Cyclin A2 Antibody (E67) [NBP2-34311]](https://resources.bio-techne.com/images/products/Cyclin-A2-Antibody-E67-Immunohistochemistry-Paraffin-NBP2-34311-img0002.jpg "Immunohistochemistry-Paraffin: Cyclin A2 Antibody (E67) [NBP2-34311]")
Immunohistochemistry-Paraffin: Cyclin A2 Antibody (E67) [NBP2-34311]
Immunohistochemistry-Paraffin: Cyclin A2 Antibody (E67) [NBP2-34311] - Formalin-fixed, paraffin-embedded human Endometrium stained with Cyclin A2 Antibody (E67).Applications for Cyclin A2 Antibody (E67)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cyclin A2
Additional Cyclin A2 Products
Product Documents for Cyclin A2 Antibody (E67)
Product Specific Notices for Cyclin A2 Antibody (E67)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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