Cyclin B1 Antibody (1Q3E9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15626

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15626-100ul
NBP3-15626-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1Q3E9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cyclin B1 Antibodies »

Product Summary for Cyclin B1 Antibody (1Q3E9)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 3-177 of human Cyclin B1 (P14635). LRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclin B1 Antibody (1Q3E9)

Western Blot: Cyclin B1 Antibody (1Q3E9) [NBP3-15626]

Western Blot: Cyclin B1 Antibody (1Q3E9) [NBP3-15626]

Western Blot: Cyclin B1 Antibody (1Q3E9) [NBP3-15626] - Analysis of extracts of various cell lines, using Cyclin B1 antibody (NBP3-15626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: Cyclin B1 Antibody (1Q3E9) [NBP3-15626]

Immunohistochemistry-Paraffin: Cyclin B1 Antibody (1Q3E9) [NBP3-15626]

Immunohistochemistry-Paraffin: Cyclin B1 Antibody (1Q3E9) [NBP3-15626] - Human colon carcinoma using [KO Validated] Cyclin B1 Rabbit mAb (NBP3-15626) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Knockout Validated: Cyclin B1 Antibody (1Q3E9) [NBP3-15626]

Knockout Validated: Cyclin B1 Antibody (1Q3E9) [NBP3-15626]

Knockout Validated: Cyclin B1 Antibody (1Q3E9) [NBP3-15626] - Western blot analysis of extracts from normal (control) and Cyclin B1 knockout (KO) HeLa cells, using Cyclin B1 antibody (NBP3-15626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
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Applications for Cyclin B1 Antibody (1Q3E9)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cyclin B1

Alternate Names

CCNB1

Gene Symbol

CCNB1

Additional Cyclin B1 Products

Product Documents for Cyclin B1 Antibody (1Q3E9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cyclin B1 Antibody (1Q3E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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