Cyclin B1 Antibody (5G6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51669

Novus Biologicals, part of Bio-Techne
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NBP1-51669
NBP1-51669-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G6

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Cyclin B1 Antibodies »

Product Summary for Cyclin B1 Antibody (5G6) - BSA Free

Immunogen

Purified recombinant fragment of human Cyclin B1 expressed in E. Coli.

Marker

Mitosis Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cyclin B1 Antibody (5G6) - BSA Free

Western Blot: Cyclin B1 Antibody (5G6)BSA Free [NBP1-51669]

Western Blot: Cyclin B1 Antibody (5G6)BSA Free [NBP1-51669]

Western Blot: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Cyclin B1 Antibody (5G6) - BSA Free [NBP1-51669]

Immunocytochemistry/ Immunofluorescence: Cyclin B1 Antibody (5G6) - BSA Free [NBP1-51669]

Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis of Hela cells using CCNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry: Cyclin B1 Antibody (5G6) - BSA Free [NBP1-51669]

Flow Cytometry: Cyclin B1 Antibody (5G6) - BSA Free [NBP1-51669]

Flow Cytometry: Cyclin B1 Antibody (5G6) [NBP1-51669] - Analysis of Hela cells using CCNB1 mouse mAb (green) and negative control (purple).

Applications for Cyclin B1 Antibody (5G6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclin B1

In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is Ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Alternate Names

CCNB1

Gene Symbol

CCNB1

Additional Cyclin B1 Products

Product Documents for Cyclin B1 Antibody (5G6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin B1 Antibody (5G6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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