Cyclin B1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-56867
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody [NBP2-56867]](https://resources.bio-techne.com/images/products/Cyclin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-56867-img0007.jpg "Immunohistochemistry-Paraffin: Cyclin B1 Antibody [NBP2-56867]")
![Immunohistochemistry: Cyclin B1 Antibody [NBP2-56867]](https://resources.bio-techne.com/images/products/Cyclin-B1-Antibody-Immunohistochemistry-NBP2-56867-img0004.jpg "Immunohistochemistry: Cyclin B1 Antibody [NBP2-56867]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Cyclin B1 Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: TRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cyclin B1 Antibody
![Western Blot: Cyclin B1 Antibody [NBP2-56867]](https://resources.bio-techne.com/images/products/Cyclin-B1-Antibody-Western-Blot-NBP2-56867-img0003.jpg "Western Blot: Cyclin B1 Antibody [NBP2-56867]")
Western Blot: Cyclin B1 Antibody [NBP2-56867]
Western Blot: Cyclin B1 Antibody [NBP2-56867] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.![Immunocytochemistry/ Immunofluorescence: Cyclin B1 Antibody [NBP2-56867]](https://resources.bio-techne.com/images/products/Cyclin-B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56867-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Cyclin B1 Antibody [NBP2-56867]")
Immunocytochemistry/ Immunofluorescence: Cyclin B1 Antibody [NBP2-56867]
Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody [NBP2-56867] - Staining of human cell line A549 shows localization to cytosol.![Immunohistochemistry-Paraffin: Cyclin B1 Antibody [NBP2-56867]](https://resources.bio-techne.com/images/products/Cyclin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-56867-img0005.jpg "Immunohistochemistry-Paraffin: Cyclin B1 Antibody [NBP2-56867]")
![Immunohistochemistry-Paraffin: Cyclin B1 Antibody [NBP2-56867]](https://resources.bio-techne.com/images/products/Cyclin-B1-Antibody-Immunohistochemistry-Paraffin-NBP2-56867-img0006.jpg "Immunohistochemistry-Paraffin: Cyclin B1 Antibody [NBP2-56867]")
Applications for Cyclin B1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cyclin B1
Alternate Names
CCNB1
Gene Symbol
CCNB1
Additional Cyclin B1 Products
Product Documents for Cyclin B1 Antibody
Product Specific Notices for Cyclin B1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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