Cyclin B2 Antibody (1L9B9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16663

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16663-100ul
NBP3-16663-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1L9B9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cyclin B2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cyclin B2 (O95067). MALLRRPTVSSDLENIDTGVNSKVKSHVTIRRTVLEEIGNRVTTRAAQVAKKAQNTKVPVQPTKTTNVNKQLKPTASVKPVQMEKLAPKGPSPTPEDVSM

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclin B2 Antibody (1L9B9)

Western Blot: Cyclin B2 Antibody (1L9B9) [NBP3-16663]

Western Blot: Cyclin B2 Antibody (1L9B9) [NBP3-16663]

Western Blot: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Western blot analysis of extracts of Rat testis cells, using Cyclin B2 antibody (NBP3-16663) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Cyclin B2 Antibody (1L9B9) [NBP3-16663]

Immunocytochemistry/ Immunofluorescence: Cyclin B2 Antibody (1L9B9) [NBP3-16663]

Immunocytochemistry/Immunofluorescence: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Immunofluorescence analysis of NIH-3T3 cells using Cyclin B2 Rabbit mAb (NBP3-16663) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Cyclin B2 Antibody (1L9B9) [NBP3-16663]

Immunohistochemistry-Paraffin: Cyclin B2 Antibody (1L9B9) [NBP3-16663]

Immunohistochemistry-Paraffin: Cyclin B2 Antibody (1L9B9) [NBP3-16663] - Immunohistochemistry of paraffin-embedded human liver cancer using Cyclin B2 Rabbit mAb (NBP3-16663) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for Cyclin B2 Antibody (1L9B9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cyclin B2

Cyclin B2 (also CCNB2 and G2/mitotic-specific cyclin-B2) is a 45-46 kDa member of the Cyclin AB subfamily, cyclin family of proteins. It is widely expressed and associates with CDK1 providing substrate specificity to a phosphorylating complex. A phosphorCDK1:Cyclin B2 complex is associated with the Golgi apparatus, where it contributes to Golgi fragmentation during mitosis. It is also possible that Cyclin B2 can substitute for Cyclin B1 during the early stages of mitosis. Human Cyclin B2 is 398 amino acids (aa) in length. It contains two cyclin box folds (aa 201-290 and 298-383) and two substrate binding sites (aa 165-254 and 264-347). Phosphorylation occurs at Ser92, Thr94, Ser99, Ser204, Ser392 and Ser398. Over aa 1-101, human Cyclin B2 shares 79% aa identity with mouse Cyclin B2.

Alternate Names

CCNB2

Gene Symbol

CCNB2

Additional Cyclin B2 Products

Product Documents for Cyclin B2 Antibody (1L9B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin B2 Antibody (1L9B9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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