Cyclin B2 Antibody (S05-3A6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14921

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14921-100ul
NBP3-14921-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S05-3A6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cyclin B2 Antibodies »

Product Specifications

Immunogen

Recombinant protein of human Cyclin B2 (Uniprot#: O95067)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cyclin B2 Antibody (S05-3A6)

Western Blot: Cyclin B2 Antibody (S05-3A6) [NBP3-14921]

Western Blot: Cyclin B2 Antibody (S05-3A6) [NBP3-14921]

Western Blot: Cyclin B2 Antibody (S05-3A6) [NBP3-14921] - Western blot detection of Cyclin B2 in Hela, A549, MCF-7, U251 cell lysates using Cyclin B2 Rabbit mAb (1:1000 diluted). Predicted band size: 45kDa. Observed band size: 45kDa.

Applications for Cyclin B2 Antibody (S05-3A6)

Application
Recommended Usage

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclin B2

Cyclin B2 (also CCNB2 and G2/mitotic-specific cyclin-B2) is a 45-46 kDa member of the Cyclin AB subfamily, cyclin family of proteins. It is widely expressed and associates with CDK1 providing substrate specificity to a phosphorylating complex. A phosphorCDK1:Cyclin B2 complex is associated with the Golgi apparatus, where it contributes to Golgi fragmentation during mitosis. It is also possible that Cyclin B2 can substitute for Cyclin B1 during the early stages of mitosis. Human Cyclin B2 is 398 amino acids (aa) in length. It contains two cyclin box folds (aa 201-290 and 298-383) and two substrate binding sites (aa 165-254 and 264-347). Phosphorylation occurs at Ser92, Thr94, Ser99, Ser204, Ser392 and Ser398. Over aa 1-101, human Cyclin B2 shares 79% aa identity with mouse Cyclin B2.

Alternate Names

CCNB2

Gene Symbol

CCNB2

Additional Cyclin B2 Products

Product Documents for Cyclin B2 Antibody (S05-3A6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin B2 Antibody (S05-3A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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