Key Product Details

Species Reactivity

Human, Mouse, Rat, Xenopus

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # X29.2

Concentration

0.2 mg/ml

View all Cyclin B2 Antibodies »

Product Specifications

Immunogen

Recombinant full-length Xenopus laevis Cyclin B2 protein (Uniprot: O95067)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08843)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for Cyclin B2 Antibody (X29.2)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cyclin B2

Cyclin B2 (also CCNB2 and G2/mitotic-specific cyclin-B2) is a 45-46 kDa member of the Cyclin AB subfamily, cyclin family of proteins. It is widely expressed and associates with CDK1 providing substrate specificity to a phosphorylating complex. A phosphorCDK1:Cyclin B2 complex is associated with the Golgi apparatus, where it contributes to Golgi fragmentation during mitosis. It is also possible that Cyclin B2 can substitute for Cyclin B1 during the early stages of mitosis. Human Cyclin B2 is 398 amino acids (aa) in length. It contains two cyclin box folds (aa 201-290 and 298-383) and two substrate binding sites (aa 165-254 and 264-347). Phosphorylation occurs at Ser92, Thr94, Ser99, Ser204, Ser392 and Ser398. Over aa 1-101, human Cyclin B2 shares 79% aa identity with mouse Cyclin B2.

Alternate Names

CCNB2

Gene Symbol

CCNB2

UniProt

O95067 (Human)

Additional Cyclin B2 Products

Product Documents for Cyclin B2 Antibody (X29.2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cyclin B2 Antibody (X29.2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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