Cyclin B2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98962

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cyclin B2 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Cyclin B2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cyclin B2 Antibody

Immunocytochemistry/ Immunofluorescence: Cyclin B2 Antibody [NBP2-98962]

Immunocytochemistry/ Immunofluorescence: Cyclin B2 Antibody [NBP2-98962]

Immunocytochemistry/Immunofluorescence: Cyclin B2 Antibody [NBP2-98962] - Immunofluorescence staining of Cyclin B2 in SW480 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Cyclin B2 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cytoplasm.
Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP2-98962]

Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP2-98962]

Immunohistochemistry-Paraffin: Cyclin B2 Antibody [NBP2-98962] - Immunochemical staining of human Cyclin B2 in human adrenal gland with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Applications for Cyclin B2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclin B2

Cyclin B2 (also CCNB2 and G2/mitotic-specific cyclin-B2) is a 45-46 kDa member of the Cyclin AB subfamily, cyclin family of proteins. It is widely expressed and associates with CDK1 providing substrate specificity to a phosphorylating complex. A phosphorCDK1:Cyclin B2 complex is associated with the Golgi apparatus, where it contributes to Golgi fragmentation during mitosis. It is also possible that Cyclin B2 can substitute for Cyclin B1 during the early stages of mitosis. Human Cyclin B2 is 398 amino acids (aa) in length. It contains two cyclin box folds (aa 201-290 and 298-383) and two substrate binding sites (aa 165-254 and 264-347). Phosphorylation occurs at Ser92, Thr94, Ser99, Ser204, Ser392 and Ser398. Over aa 1-101, human Cyclin B2 shares 79% aa identity with mouse Cyclin B2.

Alternate Names

CCNB2

Gene Symbol

CCNB2

Additional Cyclin B2 Products

Product Documents for Cyclin B2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin B2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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