Cyclin D3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98877

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cyclin D3 Antibodies »

Product Summary for Cyclin D3 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Cyclin D3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cyclin D3 Antibody

Western Blot: Cyclin D3 Antibody [NBP2-98877]

Western Blot: Cyclin D3 Antibody [NBP2-98877]

Western Blot: Cyclin D3 Antibody [NBP2-98877] - Anti-Cyclin D3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa
Immunocytochemistry/ Immunofluorescence: Cyclin D3 Antibody [NBP2-98877]

Immunocytochemistry/ Immunofluorescence: Cyclin D3 Antibody [NBP2-98877]

Immunocytochemistry/Immunofluorescence: Cyclin D3 Antibody [NBP2-98877] - Immunofluorescence staining of Cyclin D3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Cyclin D3 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.

Applications for Cyclin D3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclin D3

During the G1 phase of the cell cycle, a cell must "choose" between rest and cell division. A small subfamily of cyclin family molecules, termed cyclin D proteins (D1, D2, D3), promotes commitment to mitosis. They accomplish this by interacting with cyclin-dependent kinases (cdk4 and cdk6) to generate Ser/Thr kinase complexes that remove blocks on DNA synthesis.

Alternate Names

CCND3

Gene Symbol

CCND3

Additional Cyclin D3 Products

Product Documents for Cyclin D3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cyclin D3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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