Cyclin E1 Antibody (3A5)

Immunohistochemistry: Cyclin E1 Antibody (3A5) [NBP3-26692] -

Immunohistochemistry: Cyclin E1 Antibody (3A5) [NBP3-26692] - Image of Cyclin E1 Antibody (3A5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunohistochemistry: Cyclin E1 Antibody (3A5) [NBP3-26692] -

Immunohistochemistry: Cyclin E1 Antibody (3A5) [NBP3-26692] - Image of Cyclin E1 Antibody (3A5) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunocytochemistry/Immunofluorescence: Cyclin E1 Antibody (3A5) [NBP3-26692] -

Immunocytochemistry/Immunofluorescence: Cyclin E1 Antibody (3A5) [NBP3-26692] - Staining of Hela Cells with Cyclin E1 Antibody (3A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).