Cyclin E1 Antibody (5F8C5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37247

Novus Biologicals, part of Bio-Techne
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NBP2-37247
NBP2-37247-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5F8C5

Format

BSA Free

Concentration

1 mg/ml

View all Cyclin E1 Antibodies »

Product Summary for Cyclin E1 Antibody (5F8C5) - BSA Free

Immunogen

Purified recombinant fragment of human Cyclin E1 (AA: 307-410) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cyclin E1 Antibody (5F8C5) - BSA Free

Western Blot: Cyclin E1 Antibody (5F8C5)BSA Free [NBP2-37247]

Western Blot: Cyclin E1 Antibody (5F8C5)BSA Free [NBP2-37247]

Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mouse mAb against Hela (1), K562 (2), NIH/3T3 (3), C6 (4), MCF-7 (5), Jurkat (6), A431 (7) cell lysate.
Flow Cytometry: Cyclin E1 Antibody (5F8C5) - BSA Free [NBP2-37247]

Flow Cytometry: Cyclin E1 Antibody (5F8C5) - BSA Free [NBP2-37247]

Flow Cytometry: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Flow cytometric analysis of K652 cells using CCNE1 mouse mAb (green) and negative control (red).
Western Blot: Cyclin E1 Antibody (5F8C5)BSA Free [NBP2-37247]

Western Blot: Cyclin E1 Antibody (5F8C5)BSA Free [NBP2-37247]

Western Blot: Cyclin E1 Antibody (5F8C5) [NBP2-37247] - Western blot analysis using CCNE1 mAb against human CCNE1 (AA: 307-410) recombinant protein. (Expected MW is 37.5 kDa)
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Applications for Cyclin E1 Antibody (5F8C5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclin E1

Alternate Names

CCNE, CCNE1

Gene Symbol

CCNE1

Additional Cyclin E1 Products

Product Documents for Cyclin E1 Antibody (5F8C5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin E1 Antibody (5F8C5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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