Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08805
![Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free [NBP3-08805]](https://resources.bio-techne.com/images/products/Cyclin-E1-Antibody-CCNE1-2460-Immunohistochemistry-Paraffin-NBP3-08805-img0002.jpg "Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free [NBP3-08805]")
![Protein Array: Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free [NBP3-08805]](https://resources.bio-techne.com/images/products/Cyclin-E1-Antibody-CCNE1-2460-Protein-Array-NBP3-08805-img0004.jpg "Protein Array: Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free [NBP3-08805]")
![Western Blot: Cyclin E1 Antibody (CCNE1/2460)Azide and BSA Free [NBP3-08805]](https://resources.bio-techne.com/images/products/Cyclin-E1-Antibody-CCNE1-2460-Western-Blot-NBP3-08805-img0003.jpg "Western Blot: Cyclin E1 Antibody (CCNE1/2460)Azide and BSA Free [NBP3-08805]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # CCNE1/2460
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free
Immunogen
Recombinant human Cyclin E1 protein fragment (around aa 10-176) (exact sequence is proprietary) (Uniprot: IDP24864)
Localization
Nuclear
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07484).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free
Western Blot: Cyclin E1 Antibody (CCNE1/2460)Azide and BSA Free [NBP3-08805]
Western Blot: Cyclin E1 Antibody (CCNE1/2460) [NBP3-08805] - Western Blot Analysis of human Jurkat cell lysate using Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).![Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free [NBP3-08805]](https://resources.bio-techne.com/images/products/Cyclin-E1-Antibody-CCNE1-2460-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08805-img0005.jpg "Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free [NBP3-08805]")
Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free [NBP3-08805]
Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free [NBP3-08805] - Cyclin E1 stained with CODEX in tonsil tissue. Image from verified customer review.![Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free [NBP3-08805]](https://resources.bio-techne.com/images/products/Cyclin-E1-Antibody-CCNE1-2460-Immunohistochemistry-Paraffin-NBP3-08805-img0001.jpg "Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free [NBP3-08805]")
Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free [NBP3-08805]
Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/2460) [NBP3-08805] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cyclin E1 Mouse Monoclonal Antibody (CCNE1/2460).Applications for Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NBP3-08805 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Cyclin E1
Alternate Names
CCNE, CCNE1
Gene Symbol
CCNE1
Additional Cyclin E1 Products
Product Documents for Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free
Product Specific Notices for Cyclin E1 Antibody (CCNE1/2460) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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