Cyclin H Antibody (3N5S3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16325

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16325-100ul
NBP3-16325-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3N5S3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cyclin H Antibodies »

Product Summary for Cyclin H Antibody (3N5S3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 224-323 of human Cyclin H (P51946). AGITMESYLSESLMLKENRTCLSQLLDIMKSMRNLVKKYEPPRSEEVAVLKQKLERCHSAELALNVITKKRKGYEDDDYVSKKSKHEEEEWTDDDLVESL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclin H Antibody (3N5S3)

Western Blot: Cyclin H Antibody (3N5S3) [NBP3-16325]

Western Blot: Cyclin H Antibody (3N5S3) [NBP3-16325]

Western Blot: Cyclin H Antibody (3N5S3) [NBP3-16325] - Western blot analysis of extracts of various cell lines, using Cyclin H Rabbit mAb (NBP3-16325) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: Cyclin H Antibody (3N5S3) [NBP3-16325]

Immunocytochemistry/ Immunofluorescence: Cyclin H Antibody (3N5S3) [NBP3-16325]

Immunocytochemistry/Immunofluorescence: Cyclin H Antibody (3N5S3) [NBP3-16325] - Immunofluorescence analysis of U-2 OS cells using Cyclin H Rabbit mAb (NBP3-16325) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Cyclin H Antibody (3N5S3) [NBP3-16325]

Immunohistochemistry-Paraffin: Cyclin H Antibody (3N5S3) [NBP3-16325]

Immunohistochemistry-Paraffin: Cyclin H Antibody (3N5S3) [NBP3-16325] - Immunohistochemistry of paraffin-embedded mouse testis using Cyclin H Rabbit mAb (NBP3-16325) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Cyclin H Antibody (3N5S3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cyclin H

Alternate Names

CAK, cyclin H, cyclin-dependent kinase-activating kinase complex subunit, cyclin-H, MO15-associated protein, p34CAK complex subunit, p37CDK-activating kinase complex subunit

Gene Symbol

CCNH

Additional Cyclin H Products

Product Documents for Cyclin H Antibody (3N5S3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin H Antibody (3N5S3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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