Cyclin T1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05509

Novus Biologicals, part of Bio-Techne
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NBP3-05509-100ul
NBP3-05509-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cyclin T1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KTSEQTILNMISQSSSDTTIAGLMSMSTSTTSAVPSLPVSEESSSNLTSVEMLPGKRWLSSQPSFKLEPTQGHRTSENLALTGVDHSLPQDGSNAFISQKQNSKSVPSAKVSLKEYRAKHAEELAAQKRQLEN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclin T1 Antibody

Western Blot: Cyclin T1 Antibody [NBP3-05509]

Western Blot: Cyclin T1 Antibody [NBP3-05509]

Western Blot: Cyclin T1 Antibody [NBP3-05509] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CCNT1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: Cyclin T1 Antibody [NBP3-05509]

Western Blot: Cyclin T1 Antibody [NBP3-05509]

Western Blot: Cyclin T1 Antibody [NBP3-05509] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Cyclin T1 Antibody [NBP3-05509]

Immunocytochemistry/ Immunofluorescence: Cyclin T1 Antibody [NBP3-05509]

Immunocytochemistry/Immunofluorescence: Cyclin T1 Antibody [NBP3-05509] - Staining of human cell line U-251 MG shows localization to nucleoplasm.
View 4 more images

Applications for Cyclin T1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF fixation permeabilization use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclin T1

Cyclin T1 belongs to the cyclin family and is a primary regulator of CDK kinases. More specifically, this cyclin associates with CDK9 complex, also known as transcription elongation factor B (P-TEFb). This complex allows for the transcription of viral genes and is also involved in the regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit. Cyclin interacts with HIV-1, HIV-2, Tat and has a high affinity to TAR.

Alternate Names

CCNT, CCNT1, CCNTcyclin-T, CDK9-associated C-type protein, cyclin C-related protein, cyclin T1, cyclin T1b, Cyclin-T, cyclin-T1, CYCT1, HIVE1, human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1, Human immunodeficiency virus-1 expression, subunit of positive elongation transcription factor b

Gene Symbol

CCNT1

Additional Cyclin T1 Products

Product Documents for Cyclin T1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cyclin T1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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