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Cyclin T1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13224

Novus Biologicals, part of Bio-Techne
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NBP3-13224

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclin T1. The exact sequence is proprietary.

Reactivity Notes

Mouse (86%), Rat (87%), Bovine (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Cyclin T1 Antibody

Western Blot: Cyclin T1 Antibody [NBP3-13224]

Western Blot: Cyclin T1 Antibody [NBP3-13224]

Western Blot: Cyclin T1 Antibody [NBP3-13224] - Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cyclin T1 antibody (NBP3-13224) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Cyclin T1 Antibody [NBP3-13224]

Immunocytochemistry/ Immunofluorescence: Cyclin T1 Antibody [NBP3-13224]

Immunocytochemistry/Immunofluorescence: Cyclin T1 Antibody [NBP3-13224] - Cyclin T1 antibody detects Cyclin T1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin T1 protein stained by Cyclin T1 antibody (NBP3-13224) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Cyclin T1 Antibody

Western Blot: Cyclin T1 Antibody [NBP3-13224] -

Western Blot: Cyclin T1 Antibody [NBP3-13224] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cyclin T1 antibody (NBP3-13224) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Cyclin T1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclin T1

Cyclin T1 belongs to the cyclin family and is a primary regulator of CDK kinases. More specifically, this cyclin associates with CDK9 complex, also known as transcription elongation factor B (P-TEFb). This complex allows for the transcription of viral genes and is also involved in the regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit. Cyclin interacts with HIV-1, HIV-2, Tat and has a high affinity to TAR.

Alternate Names

CCNT, CCNT1, CCNTcyclin-T, CDK9-associated C-type protein, cyclin C-related protein, cyclin T1, cyclin T1b, Cyclin-T, cyclin-T1, CYCT1, HIVE1, human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1, Human immunodeficiency virus-1 expression, subunit of positive elongation transcription factor b

Gene Symbol

CCNT1

Additional Cyclin T1 Products

Product Documents for Cyclin T1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cyclin T1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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