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Cyclophilin A Antibody (1F4-1B5)

Novus Biologicals, part of Bio-Techne | Catalog # H00005478-M01

Novus Biologicals, part of Bio-Techne
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H00005478-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F4-1B5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PPIA (AAH00689.1, 1 a.a. - 165 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITIADCGQLE

Specificity

PPIA - peptidylprolyl isomerase A (cyclophilin A)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Cyclophilin A Antibody (1F4-1B5)

Western Blot: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01]

Western Blot: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01]

Western Blot: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01] - PPIA monoclonal antibody (M01), clone 1F4-1B5 Analysis of PPIA expression in Jurkat.
Western Blot: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01]

Western Blot: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01]

Western Blot: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01] - Analysis of PPIA expression in transfected 293T cell line by PPIA monoclonal antibody (M01), clone 1F4-1B5.Lane 1: PPIA transfected lysate(18 KDa).Lane 2: Non-transfected lysate.
ELISA: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01]

ELISA: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01]

ELISA: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01] - Detection limit for recombinant GST tagged PPIA is approximately 0.03ng/ml as a capture antibody.

Applications for Cyclophilin A Antibody (1F4-1B5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cyclophilin A

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.

Alternate Names

CYPA, CYPH, PPIA, Rotamase

Entrez Gene IDs

5478 (Human)

Gene Symbol

PPIA

OMIM

123840 (Human)

Additional Cyclophilin A Products

Product Documents for Cyclophilin A Antibody (1F4-1B5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cyclophilin A Antibody (1F4-1B5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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