Cyclophilin B Antibody (CL3901)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59781

Novus Biologicals, part of Bio-Techne
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NBP2-59781
NBP2-59781-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL3901

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cyclophilin B Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Cyclophilin B Antibody (CL3901)

Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis in human placenta and skeletal muscle tissues using NBP2-59781 antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.
Western Blot: Cyclophilin B Antibody (CL3901) [NBP2-59781]

Western Blot: Cyclophilin B Antibody (CL3901) [NBP2-59781]

Western Blot: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-PPIB monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
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Applications for Cyclophilin B Antibody (CL3901)

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclophilin B

Cyclophilin B (SCYLP, CyPB and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, Cyclophilin B mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human Cyclophilin B precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27-34), a PPIase domain (aa 47-204) and a C-terminal ER retention motif (aa 213-216). Over aa 34-216, the human and mouse sequences are 95% aa identical.

Alternate Names

CYP-S1, CYPB, PPIB, S-cyclophilin, SCYLP

Gene Symbol

PPIB

Additional Cyclophilin B Products

Product Documents for Cyclophilin B Antibody (CL3901)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cyclophilin B Antibody (CL3901)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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